PENGARUH PEMBERIAN EKSTRAK ETANOL ALGA COKELAT (Sargassum sp.) TERHADAP HITUNG JENIS LEUKOSIT MENCIT (Mus musculus) PASCA DIINDUKSI STRES AKUT

Abstract

Glucocorticoids are stress hormones that suppress normal immune responses by blocking Th1 cells. Glucocorticoids have an impact on decreasing the body's immune system and changes in leukocyte differentiation. Sargassum sp. is one type of  brown  algae  that  lives  in  the  sea.  Sargassum  sp.  contains  metabolite  compounds  that  are  immunomodulatory.  This study aims to determine the number of types of leukocytes in mice that are induced by stress and given ethanol extract of Sargassum sp. Pure experimental research design with post test only control group design. Thirty mice were divided into five  treatment  groups,  namely  the  normal  control  group  (KN),  negative  control  (K-),  positive  control  (K  +),  ethanol extract of Sargassum sp. 35% (P1) and 70% (P2). At K-, K +, P1 and P2 were given stress treatment with the FST (Forced Swim  Test)  method  to  see  immobility  time  in  mice.  The  treatment  was  given  for  seven  days  and  on  the  8th  day  the intracardial mice were taken. Observation of leukocytes from the blood smear of mice was carried out using a microscope with  1000x  magnification.  Observation  data  were  analyzed  by  ANOVA.  The  results  showed  that  the  P1  group  could reduce  the  percentage  of  neutrophils  and  monocytes  but  increase  the  percentage  of  lymphocytes,  while  the  P2  group showed a decrease in the percentage of neutrophils, an increase in the percentage of lymphocytes, but could not decrease the  percentage  of  monocytes  compared  to  P1.  The  mean  percentage  of  eosinophils  and  basophils  did  not  change significantly, both in the treatment group and in the control group. However, statistical data shows no significant change between  groups.  So  it  can  be  concluded  that  stress  treatment  given  to  mice  does  not  have  a  significant  effect  on  the average percentage of monocytes, lymphocytes, neutrophils, eosinophils and basophils after being induced by stress.