Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus

Abstract

In this paper, we successfully constructed the cDNA library of major ampullate gland of Araneus ventricousus using pUC18 vector and cloned the partial cDNA (AvMaSp1, GenBank accession number AY177203) encoding spider major ampullate gland spidroin-1 by means of picking colony randomly (Bird gun). The partial cDNA sequence of AvMaSp1 was 1 408 bp and its region in encoding was 1 288 bp. The protein deduced from AvMaSp1 contained 429 amino acid residues and molecular weight was 34.07 kDa. The repetitive motif of this cDNA sequence was (GA)nAm(GA)N and the identity with A. diadematus fibroin-1 mRNA (ADF-1, GenBank accession number ADU47853) was 75.0%.