Cell Cycle Dynamics in Glioma Cancer Stem Cells

Abstract

Cancer stem cells, sometimes referred to as tumor initiating cells, play pivotal roles in tumor initiation, progression, metastasis, resistance to therapy, and relapse. Understanding how these populations of cells expand in response to a host of conditions is critical in determining effective cancer therapeutics. A defining feature of cancer stem cells is the ability to switch between modes of quiescence and symmetric/asymmetric division to protect and conserve the population, this feature is traditionally reserved for normal adult stem cell populations. Understanding how the core cell cycle machinery responds to external cues to drive symmetric/asymmetric division vs. quiescence will reveal fundamental information about how cancer stem cell populations survive and expand. This chapter will describe methods to study the cell cycle dynamics in brain cancer stem cell populations and how they compare to the other populations in a tumor.