Nucleolar isolation allows exhaustive characterization of the nucleolar content. Centrifugation-based protocols are not adapted to isolation of nucleoli directly from a plant tissue because of copurification of cellular debris. We describe here a method that allows the purification of nucleoli using fluorescent-activated cell sorting from Arabidopsis thaliana leaves. This approach requires the expression of a specific nucleolar protein such as fibrillarin fused to green fluorescent protein in planta .
CITATION STYLE
Pontvianne, F., Boyer-Clavel, M., & Sáez-Vásquez, J. (2016). Fluorescence-activated nucleolus sorting in Arabidopsis. In Methods in Molecular Biology (Vol. 1455, pp. 203–211). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3792-9_15
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