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Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy

  • Enderlein J
Springer US, (2009), 243-263
DOI: 10.1007/978-0-387-76497-9_9
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Abstract

The chapter presents a general introduction to the method of fluorescence correlation spectroscopy (FCS) and its recentlydeveloped modification, dual-focus FCS. It explains the general optical setup of an FCS system, data acquisition, and dataanalysis. It discusses numerous potential optical and photophysical artifacts of FCS measurements and explains how these problemsare circumvented by dual-focus FCS.

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Enderlein, J. (2009). Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy. In Handbook of Single-Molecule Biophysics (pp. 243–263). Springer US. https://doi.org/10.1007/978-0-387-76497-9_9

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