The bacterial expression of glycoproteins has experienced significant progress in recent years, particularly in regard to the production of conjugate vaccines against pathogens. In this case, a protein carrier conjugated with glycosides is used to produce intense stimulation of the immune system. Glycoconjugate vaccines account for 35% of the global vaccine market, and consequently, several biotechnological companies have developed products for the purification of glycosylated proteins to attain homogeneity. In this chapter we present a general process for glycoprotein production in Escherichia coli and a practice method for purification of glycosylated proteins, using affinity chromatography.
CITATION STYLE
Breyer, C. A., de Oliveira, M. A., & Pessoa, A. (2018). Expression of glycosylated proteins in bacterial system and purification by affinity chromatography. In Methods in Molecular Biology (Vol. 1674, pp. 183–191). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7312-5_14
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