Metabolism of 5 hydroxylysine in Pseudomonas fluorescens

Abstract

Hydroxylysine is metabolized via two routes by a P. fluorescens strain as shown by the oxidation of selected intermediates. Hydroxy L lysine is oxidized via a pathway analogous to the monooxygenase pathway for L lysine, and data suggest that at least some of the enzymes are those involved in the metabolism of L lysine. Hydroxy L lysine is also converted by a racemase to allohydroxy D lysine, which is then degraded via a pathway analogous to, but different from, that described for D lysine, involving hydroxy L pipecolate, 2 amino 5 hydroxyadipate, and 2 hydroxyglutarate. Data obtained with mutants unable to oxidize L pipecolate suggest that the enzymes for the metabolism of hydroxy L pipecolate are distinct from those for L pipecolate. Studies on D and L lysine degradation have shown that the previously described pathways for these compounds are present in this soil pseudomonad.