Abstract
Macroautophagy, by its very nature, is a protein trafficking process. Cargos are transported and processed. Atg proteins come and go. In this chapter, we present three assays to monitor these dynamic events: a non-radioactive pulse-chase labeling assay to monitor the transport of prApe1 and two fluorescent microscopy-based assays to assess the trafficking of Atg8 and Atg9.
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APA
Zhu, J., & Xie, Z. (2021). Assays for autophagy iii: Observing dynamic protein trafficking. In Methods in Molecular Biology (Vol. 2196, pp. 211–222). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0868-5_16
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