Hyperglycemia in apolipoprotein E-deficient mouse strains with different atherosclerosis susceptibility

Abstract

Background: Type 2 diabetes mellitus (T2DM) is associated with an increased risk of atherosclerotic vascular disease, but it is unknown whether the other way around is true too. C57BL/6 (B6) and BALB/cJ (BALB) are two mouse strains that differ markedly in their susceptibility to atherosclerosis. In this study we investigated the development of diet-induced T2DM in these two strains.Methods and Results: When deficient in apolipoprotein E (apoE -/-) and fed a Western diet for 12 weeks, atherosclerosis-susceptible B6 mice developed significant hyperglycemia. In contrast, atherosclerosis-resistant BALB apoE -/-mice had much lower plasma glucose levels than B6.apoE -/-mice on either chow or Western diet and during an intraperitoneal glucose tolerance test. In response to glucose BALB.apoE -/-mice displayed both the first and second phases of insulin secretion but the second phase of insulin secretion was absent in B6.apoE -/-mice. In response to insulin B6.apoE -/-mice showed a deeper and longer-lasting fall in blood glucose levels while BALB.apoE -/-mice showed little reduction in glucose levels. Pancreatic islet area of BALB.apoE -/-mice on light microscopy nearly doubled the area of B6.apoE -/-mice. Most circulating proinflammatory cytokines were lower in BALB.apoE -/-than in B6.apoE -/-mice on the Western diet, as determined by protein arrays. Increased macrophage infiltration in islets was observed in B6.apoE -/-mice by immunostaining for Mac2 and also by flow cytometry.Conclusion: This study demonstrates that defects in insulin secretion rather than defects in insulin resistance explain the marketed difference in susceptibility to T2DM in the B6.apoE -/-and BALB.apoE -/-mouse model. A smaller islet mass and more prominent islet inflammation may explain the vulnerability of B6.apoE -/-mice to diet-induced diabetes. © 2011 Li et al; licensee BioMed Central Ltd.