Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp

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Abstract

A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.

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Saul, D. J., Williams, L. C., Reeves, R. A., Gibbs, M. D., & Bergquist, P. L. (1995). Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp. Applied and Environmental Microbiology, 61(11), 4110–4113. https://doi.org/10.1128/aem.61.11.4110-4113.1995

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