To investigate mechanical-dependent bone remodeling, we had previously applied various types of mechanical loading onto the teeth of rats and mice. In vitro cultured bone cells were then used to elucidate the mechanisms underlying the specifi c phenomenon revealed by in vivo experiments. This review describes the techniques used to upregulate CCN2 expression in bone cells produced by different types of mechanical stress, such as fl uid shear stress and substrate strain in vitro, and compression or tension force in vivo.
CITATION STYLE
Takano-Yamamoto, T., Fukunaga, T., & Takeshita, N. (2017). Gene expression analysis of CCN protein in bone under mechanical stress. In Methods in Molecular Biology (Vol. 1489, pp. 283–308). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6430-7_26
Mendeley helps you to discover research relevant for your work.