Abstract
Studies of assembly and secretion of gel-forming mucins are complex. The pulse-chase methods for mucins described here include metabolic radiolabeling and labeling in animals with azido-GalNAc. The labeled mucins are analyzed by composite agarose-polyacrylamide gel electrophoresis and autoradiography or by mucus-preserving tissue fixation and Click-iT® chemistry. © 2012 Springer Science+Business Media, LLC.
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Johansson, M. E. V., & Hansson, G. C. (2012). Analysis of assembly of secreted mucins. Methods in Molecular Biology, 842, 109–121. https://doi.org/10.1007/978-1-61779-513-8_6
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