Respiratory syncytial virus (RSV) is an enveloped, nonsegmented negative-strand RNA virus, which causes lower respiratory tract infections and is a leading cause of mortality in young infants. There is no available RSV vaccine and currently administered prophylactic antibodies are limited to high-risk populations. Current efforts to develop vaccines include development of live-attenuated RSV candidates. We describe here methods for preparation and recovery of recombinant RSV using an efficient bacterial artificial chromosome (BAC)-based system, expansion and plaque purification of recovered virus, and generation of master and working stocks.
CITATION STYLE
Stobart, C. C., Hotard, A. L., Meng, J., & Moore, M. L. (2017). BAC-based recovery of recombinant respiratory syncytial virus (RSV). In Methods in Molecular Biology (Vol. 1602, pp. 111–124). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6964-7_8
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