The heat resistance of spores of Clostridium botulinum 213B heated at 121-130°C in acidified mushroom extract

Abstract

Spores of Clostridium botulinum 213B were heated in mushroom extract acidified to pH 6 with citric acid or gluconodeltalactone at temperatures of 121.1, 125, and 130°C using a thermoresistometer. Decimal reduction times were similar in acidified and natural pH (6.7) mushroom extract. At 121.1, 125, and 130°C, D values were in the range 2.44 - 2.55 s, 0.91-1.45 s, and 0.51- 0.75 s, respectively. There was no evidence that mild acidification reduced thermal resistance at high temperatures.