Determination of Total Tannins and Antibacterial Activities Ethanol Extraction Seri (Muntingia calabura L.) Leaves

Abstract

This research aims were to determine the total tannin level and to test the antibacterial activity of the ethanol extract of Muntingia calabura L., leaves. The extraction was carried out by maceration method with ethanol solvent. Determination of total tannin content was carried out by colorimetric method which was measured at a wavelength of 745 nm using UV-Vis spectrometry and tannic acid was used as the standard. The antibacterial activity test was carried out by diffusion disc method against, such as Escherichia coli, Salmonella typhi and Propionebacterium acnes bacteria. The variations in the concentration of the ethanol extract of M. calabura included 12.5%; 25%; 50%, and 75%. Chloramphenicol was used as positive control and DMSO 10% as negative control. The results showed that the ethanol extract of the M. calabura leaves had a total tannin level of 0.0655±0.0002 mg/g d.w (Mean±SD). Antibacterial activity of ethanol extraction by the M. calabura leaves against E. coli (14.18±0.96; 15.53±0.40; 15.92±1.27; and 16.50±0.52), S. typhi (13.37±0.35; 14.47±1.14; 14.97±0.87; and 15.50±0.66), and P. acnes (14.13±0.24; 14.60±0.20; 15.52±1.14; and 16.37±0.46). Antibacterial activity of Chloramphenicol against E. coli, S. typhi and P. acnes are 31.25±2.08; 25.15±1.61; and 23.25±4.42.