Immunoreactivity for choline acetyltransferase of peripheral-type (pChAT) in the trigeminal ganglion neurons of the non-human primate Macaca fascicularis

13Citations
Citations of this article
19Readers
Mendeley users who have this article in their library.

Abstract

Transcripts of the choline of acetyltransferase (ChAT) gene reveal a number of different splice variants including ChAT of a peripheral type (pChAT). Immunohistochemical staining of the brain using an antibody against pChAT clearly revealed peripheral cholinergic neurons, but failed to detect cholinergic neurons in the central nervous system. In rodents, pChATimmunoreactivity has been detected in cholinergic parasympathetic postganglionic and enteric ganglion neurons. In addition, pChAT has been observed in non-cholinergic neurons such as peripheral sensory neurons in the trigeminal and dorsal root ganglia. The common type of ChAT (cChAT) has been investigated in many parts of the brain and the spinal cord of non-human primates, but little information is available about the localization of pChAT in primate species. Here, we report the detection of pChAT immunoreactivity in trigeminal ganglion (TG) neurons and its co-localization with Substance P (SP) and/or calcitonin generelated peptide (CGRP) in the cynomolgus monkey, Macaca fascicularis. Neurons positive for pChAT were observed in a rather uniform pattern in approximately half of the trigeminal neurons throughout the TG. Most pChAT-positive neurons had small or medium-sized cell bodies. Double-immunofluorescence staining showed that 85.1% of SP-positive cells and 74.0% of CGRP-positive cells exhibited pChAT immunoreactivity. Most pChAT-positive cells were part of a larger population of neurons that co-expressed SP and/or CGRP. © 2013 The Japan Society of Histochemistry and Cytochemistry.

References Powered by Scopus

Immunoreactive calcitonin gene-related peptide and substance P coexist in sensory neurons to the spinal cord and interact in spinal behavioral responses of the rat

529Citations
N/AReaders
Get full text

Vesicular acetylcholine transporter (VAChT) protein: A novel and unique marker for cholinergic neurons in the central and peripheral nervous systems

379Citations
N/AReaders
Get full text

Calcitonin gene-related peptide coexists with substance P in capsaicin sensitive neurons and sensory ganglia of the rat

345Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Ginsenoside Rg5 improves cognitive dysfunction and beta-amyloid deposition in STZ-induced memory impaired rats via attenuating neuroinflammatory responses

125Citations
N/AReaders
Get full text

Fucoxanthin Inhibits β-Amyloid Assembly and Attenuates β-Amyloid Oligomer-Induced Cognitive Impairments

117Citations
N/AReaders
Get full text

Molecular, Cellular and Circuit Basis of Cholinergic Modulation of Pain

99Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Koga, T., Bellier, J. P., Kimura, H., & Tooyama, I. (2013). Immunoreactivity for choline acetyltransferase of peripheral-type (pChAT) in the trigeminal ganglion neurons of the non-human primate Macaca fascicularis. Acta Histochemica et Cytochemica, 46(2), 59–64. https://doi.org/10.1267/ahc.12044

Readers over time

‘13‘14‘15‘17‘18‘19‘20‘21‘22‘23‘2401234

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

46%

Researcher 6

46%

Professor / Associate Prof. 1

8%

Readers' Discipline

Tooltip

Neuroscience 5

42%

Agricultural and Biological Sciences 3

25%

Medicine and Dentistry 3

25%

Biochemistry, Genetics and Molecular Bi... 1

8%

Save time finding and organizing research with Mendeley

Sign up for free
0