HPLC-DAD/TOF-MS Chemical Compounds Analysis and Evaluation of Antibacterial Activity of Aristolochia longa Root Extracts

Abstract

The present study aimed to determine the phenolic compounds of Aristolochia longa root extracts and to evaluate their antibacterial activities on multiresistant strains. Phytochemical analysis revealed the presence of flavonoids, tannins, terpenoids, and alkaloids. The HPLC-DAD analysis of A. longa extracts showed the presence of several major bioactive compounds such as ferulic acid, 4-hydroxycinnamic acid, citric acid, and quinic acid. The agar diffusion method was used for the sensitivity test, while minimal inhibitory concentration (MIC) and minimal bactericidal concentration values were determined by microdilution assay. Different tests were carried out on 3 clinical multiresistant strains and 3 reference strains. The diameter of inhibition of Staphylococcus aureus ATCC 25923 induced by the ethyl acetate fraction at 200 mg/mL was 25 ± 1 mm. Moreover, Escherichia coli ATCC 29522 showed a great sensitivity toward all the concentrations tested. The MICs of the active extracts vary between 12.5 and 100 mg/mL with a bacteriostatic effect on Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis, and S. aureus ATCC 25923.