An enzymatic assay system for D-Asp was established using D-aspartate oxidase and oxaloacetate decarboxylase. In this system, D-Asp is converted to pyruvate, which is determined fluorometrically with 1,2-diamino- 4,5-methylenedioxybenzene. This method makes possible D-Asp measurement at the micromolar level. The D-Asp contents of an edible brown alga, Hijika fusiforme, a lactic acid bacteria beverage, and pig testis were determined by the method.
CITATION STYLE
Kato, S., Ikuta, T., Hemmi, H., Takahashi, S., Kera, Y., & Yoshimura, T. (2012). Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase. Bioscience, Biotechnology and Biochemistry, 76(11), 2150–2152. https://doi.org/10.1271/bbb.120477
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