It is highly desirable to identify gene’s function in a high-throughput fashion, and the CRISPR/Cas9 system has been harnessed to meet such a need. Here, we describe a general method to generate genome-scale lentiviral single-guide RNA (sgRNA) library and conduct a pooled function-based screening in human cells. This protocol would be of interest to researchers to rapidly identify genes in a variety of biological processes.
CITATION STYLE
Zhu, S., Zhou, Y., & Wei, W. (2017). Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells. In Methods in Molecular Biology (Vol. 1656, pp. 175–181). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7237-1_11
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