G protein-coupled receptors (GPCR) are integral membrane proteins that regulate multiple cellular processes. To obtain insights into structural properties of GPCR and mechanism of activity, these proteins should be isolated in significant (milligram) quantities, in a pure, homogenous, and stable form. Here we describe the expression and purification of type II human cannabinoid receptor CB2, a class A GPCR, in two different types of expression hosts: in Escherichia coli and in mammalian suspension cell culture Expi293. Our method allows preparation of milligram quantities of the purified receptors suitable for a wide array of downstream applications including high-resolution structural studies and functional assays.
CITATION STYLE
Yeliseev, A. A. (2021). Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor. In Methods in Molecular Biology (Vol. 2268, pp. 61–76). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1221-7_4
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