Research on automatic preprocessing equipment of salivary analyte for PCR inspection of coronavirus

Abstract

Polymerase chain reaction (PCR) inspection of salivary analyte is performed by pretreatment, RNA extraction setup, RNA extraction, PCR setup, and the PCR process. However, the pretreatment process is conducted manually, and it is a bottleneck to the overall process. The author created automatic preprocessing logistics and prototypes using robotic technology for the pretreatment process. A dissolving agent of saliva is poured into the salivary container, the transfer syringe is automated, and a transfer robot injects an inactivating solubilizer using a robotic hand. Ninety-six inactivated vessel units are processed for the next RNA extraction process. The automatic preprocessing equipment is successfully developed and used in the inspection at a hospital. Pretreatment efficiency is up to eight times greater compared to that with the conventional manual process. The 96 units/h inspection is made possible by a single of equipment. The developed automatic preprocessing method assures high efficiency, standardization, and safety for coronavirus inspection.