Different target genes and chimeric-gene hairpin structures affect virus resistance mediated by RNA silencing in transgenic tobacco

3Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.

Abstract

Two types of hairpin RNA (hpRNA) constructions were designed using a chimeric gene formed from two genes, the coat protein (CP) gene or the silencing suppressor gene, from the Cucumber mosaic virus (CMV) and the Potato virus Y (PVYN), respectively; one type generated a single hairpin structure, whereas the other formed a two-hairpin structure. Four constructs, pDCPSH (double CP gene fragments, single hairpin), pDCPDH (double CP gene fragments, double hairpins), pHC2bSH (two silencing suppressor gene fragments, single hairpin), and pHC2bDH (two silencing suppressor gene fragments, double hairpins), were individually introduced into tobacco plants. A transcript analysis demonstrates that the small interference RNA (siRNA) processing efficiency was greater with the double-hairpin construct than with the single-hairpin construct, although the expression of their target genes were similar. A viral resistance assay shows that the transgenic tobacco plants effectively resisted a mixed infection of CMV and Potato virus Y (PVYN) and that pDCPDH exhibited the highest silencing efficiency. The accumulation of siRNA in the inoculated transgenic plants expressing different hairpin structures was similar. A genetic analysis reveals that viral resistance in the transgenic plants was stably inherited from the T0 to T1 generation. A transcript analysis and a viral resistance assay indicate that the double-hairpin structure of the same target sequences tended to produce more siRNA before the virus inoculation and thus strengthened RNA-mediated viral resistance. © 2014 The Institute of Experimental Botany.

Cite

CITATION STYLE

APA

Xie, X., Song, Y., Liu, X., Wang, S., Zhu, C., & Wen, F. (2014). Different target genes and chimeric-gene hairpin structures affect virus resistance mediated by RNA silencing in transgenic tobacco. Biologia Plantarum, 58(3), 575–581. https://doi.org/10.1007/s10535-014-0422-9

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free