The production of alkaline protease from Aspergillus flavus DUCC K225 on rice bran containing medium

Abstract

Alkaline protease is an industrially important enzyme that can produce by Aspergillus flavus DUCC K225 an indigenous mold from lime soil Madura island. The production of alkaline protease by Aspergillus flavus DUCC K225 produced by submerged fermentation on modified Czapeks Dox medium containing rice bran as N source. The enzyme production was observed after 7th-day incubation, by measuring the protease activity at pH 8.5 and the temperature stability as well. The results showed that the enzyme activity is higher on the rice bran medium compared to the standard medium, with a value of 237,84 U/ml and 94.85 U/ml respectively. This alkaline protease enzyme produced also thermostable, with 89.3% stability value for 60 min at 40 C.