Dextran-coated charcoal immunoassay of insulin

Abstract

1. The use of dextran-coated charcoal allows a rapid, simple and relatively precise radioimmunoassay of insulin. - 2. The factors affecting the selectivity of coated charcoal for insulin and antibody-bound insulin have been examined and optimum operating conditions selected. - 3. The original method has been modified to allow the use of a commercially available antiserum. - 4. The accuracy and precision of the method have been compared with those of a double antibody technique. © 1968 Springer-Verlag.