MicroRNA-150 serves as a diagnostic biomarker and is involved in the inflammatory pathogenesis of Parkinson's disease

Abstract

Background: Dysregulation of microRNAs (miRNAs) has been reported to be involved in the neuroinflammatory pathogenesis of PD. This study aimed to investigate the serum expression of microRNA-150 (miR-150) in Parkinson's disease (PD) patients and further uncover the regulatory effect of miR-150 on neuroinflammation. Methods: Quantitative Real-Time PCR was used to measure the expression of miR-150. A receiver operating characteristic curve was applied to evaluate the diagnostic value of miR-150. The effect of miR-150 on neuroinflammation was analyzed by examining its correlation with proinflammatory cytokines and gain-of-function experiments in microglia treated with LPS. Results: Serum miR-150 expression was downregulated in PD patients compared with the healthy controls, and served as a candidate diagnostic biomarker for the screening of PD cases. Negative correlation was found between miR-150 levels and the levels of procytokines in PD patients. By the treatment of LPS, microglia BV2 cells had a reduced expression of miR-150, and the enhanced neuroinflammatory responses were inhibited by the overexpression of miR-150. AKT3 was verified as a target of miR-150 in BV2 cells. Conclusion: All the data of this study revealed that the decreased serum miR-150 serves as a potential diagnostic biomarker. The methods to increase miR-150 expression may have a beneficial effect in PD via suppressing the neuroinflammation by targeting AKT3.