A microtubule‐associated protein in maize is expressed during phytochrome‐induced cell elongation

  • Nick P
  • Lambert A
  • Vantard M
40Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Plants can adapt their shape to environmental stimuli. This response is mediated by the reorganization of cortical microtubules, a unique element of the cytoskeleton. However, the molecular base of this response has remained obscure so far. In an attempt to solve this problem, signal‐dependent changes in the pattern of microtubule‐binding proteins were analysed during coleoptile elongation in maize, that is, under the control of the plant photoreceptor phytochrome. Two putative MAPs of 100 kDa (P 100 ) and 50 kDa apparent molecular weights were identified in cytosolic extracts from non‐elongating and elongating cells. Both proteins co‐assembled with endogenous tubulin, bound to neurotubules and were immunologically related to the neural MAP τ: the P 100 protein, depending on the physiological situation, was manifest as a double band and was always found to be heat‐stable. In contrast, the 50 kDa MAP was heat‐stable only for particular tissues and physiological treatments. The P 100 protein was present in all tissues, however in a reduced amount in elongating coleoptiles. The 50 kDa MAP was expressed exclusively upon induction of phytochrome‐dependent cell elongation. As shown by immunofluorescence double‐staining, an epitope shared by both proteins colocalized with cortical microtubules in situ , but exclusively in elongating cells. In non‐elongating cells, only the nuclei were stained. Partially purified nuclei from elongating cells were enriched in P 100 , whereas the 50 kDa MAP became enriched in a partially purified plasma membrane fraction.

Cite

CITATION STYLE

APA

Nick, P., Lambert, A., & Vantard, M. (1995). A microtubule‐associated protein in maize is expressed during phytochrome‐induced cell elongation. The Plant Journal, 8(6), 835–844. https://doi.org/10.1046/j.1365-313x.1995.8060835.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free