Quantitative Estimation of Intracellular Neutral Lipids of the Yeast, Lipomyces starkeyi

Abstract

In this paper, we present a simple and sensitive method for the assay of intracellular neutral lipids of the yeast, Lipomyces starkeyi. In this method, we used a tower-shaped mixer for cell disruption and applied a diagnostic triglycerides assay kit as a sensitive color developer. This mixer could treat 14 samples at one time. No effect of pH on cell disruption was detected within the pH range of 1.6 to 6.8. Twenty min was chosen for cell disruption and for homogeneous dispersion of intracellular neutral lipids. The analytical results agreed well with those obtained by three conventional methods. The new method is simpler and of good reliability. © 1982, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.