Vascular colonization patterns in susceptible and resistant tomato cultivars inoculated with Fusarium oxysporum f.sp. lycopersici races 0 and 1

Abstract

The vascular colonization pattern of Fusarium oxysporum f.sp. lycopersici races 0 and 1 in tomato was studied in five susceptible and five resistant cultivar-fungus combinations during a 26-day period after inoculation by root immersion. Propagules spread discontinuously along the stems in all five cultivars 1 day after inoculation, irrespective of cultivar resistance. Five days later the fungus was limited to the stem bases in allcultivars. Between the fifth and 12th days, stem colonization by the fungus stopped in all cultivar-race combinations. Thereafter, the situation remained stable in resistant combinations, with inoculum distributed discontinuously, and no disease symptoms were apparent. By contrast, in the susceptible combinations a gradual upward colonization of the stems was seen such that fungal distribution was no longer discontinuous and disease symptoms appeared. These results suggest that a fungal 'incubation' period in the base of the vascular system is required before a secondary invasion of tissues occurs in susceptible genotypes. The slope of the regression line fitted between the height reached by the fungus Up the stem (y) and the time after inoculation (x) provides a measure of the horizontal (polygenic) resistance in tomato cultivars.