The HA1 genes from influenza A strains A/Puerto Rico/8/1934 H1N1 (A/PR/8/34) and A/Hatay/2004 H5N1 were each cloned in Pichia pastoris vectors in the correct reading frame with the yeast α-factor secretion signal and the C-terminus His-tag, resulting in simple, fast purification of expressed H1HA1 and H5HA1 protein from the culture medium. Mice vaccinated with the purified proteins showed robust T cell, anti-HA1 IgG responses and developed a high antibody response for hemagglutination inhibition (HI) at titer 7.6 log2. Chickens vaccinated with a dose of 200 g of H5HA1 mixed with either Montanide or Freund's adjuvants gave HI values of up to 7 log2 at the third week comparable with a licensed inactivated H5N1 vaccine.
CITATION STYLE
Nguyen, T. Q., Van, T. T. H., Lin, Y. C., Van, T. N. N., Bui, K. C., Le, Q. G., … Truong, N. H. (2014). A potential protein-based vaccine for influenza H5N1 from the recombinant HA1 domain of avian influenza A/H5N1 expressed in Pichia pastoris. Future Virology, 9(12), 1019–1031. https://doi.org/10.2217/fvl.14.93
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