Biochemical properties of tartrate-resistant acid phosphatase in serum of adults and children

Abstract

Spectrophotometry of total acid phosphatase activity in children's sera showed an average value of 22.4±2.9 and 7.4±0.8 U/liter, for the hydrolysis of p-nitrophenyl phosphate and α-naphthyl phosphate, respectively. Analyses of 'band 5b', after electrophoresis on acrylamide gel, gave even higher values. The values for children's sera were much higher than those for sera from adults. The multiplicity of acid phosphatases in sera of children and adults was studied by electrophoresis on acrylamide gel and by chromatography on CM-Sepharose. Both methods showed the major acid phosphatase in children's sera to be an acid pyrophosphatase, band 5b. Its catalytic properties are indistinguishable from the enzyme previously isolated from the spleen of leukemic reticuloendotheliosis.