Determining the concentration of oligonucleotide in biological samples such as tissue lysate and serum is essential for determining the biodistribution and pharmacokinetic profile, respectively. ELISA-based assays have shown far greater sensitivities compared to other methods such as HPLC and LC/MS. Here, we describe a novel ultrasensitive hybridization-based ELISA method for quantitating morpholino oligonucleotides in mouse tissue lysate and serum samples. The assay has a linear detection range of 5–250 pM (R2 > 0.99).
CITATION STYLE
Burki, U., & Straub, V. (2017). Ultrasensitive hybridization-based ELISA method for the determination of phosphorodiamidate morpholino oligonucleotides in biological samples. In Methods in Molecular Biology (Vol. 1565, pp. 265–277). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6817-6_22
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