In vivo Hemostatic Activity of Jatropha mollissima: A Triple-Blinded, Randomized, Controlled Trial in an Animal Model

Abstract

Objective The objective of this study was to evaluate the hemostatic activity of the sap from Jatropha mollissima (Pohl) Baill. in rats. Materials and Methods Twenty-four Wistar rats were randomized into four groups (n = 6): the JM25 and JM40 groups were treated with ethanolic extract from the sap of J. mollissima, in a concentration of 25 and 40 mg·mL 1, respectively; the MO group was treated with Monsel's solution and the control group SC with a 0.9% sodium chloride solution. Statistical Analysis Data were submitted to the Kurskal-Wallis' test, followed by Dunn's post hoc (p < 0.05). Results There was a significant reduction in the bleeding time of the group from the JM25 extract (p = 0.001) when compared with MO and SC. There were no statistically significant differences between groups JM25 and JM40 (p > 0.05). The JM25 group did not present rebleeding, a result significantly different from the MO group (p = 0.001). Monsel's solution showed significant bleeding, six times greater than the control group SC. Conclusion The J. mollissima extract, in the concentration of 25 mg·mL 1, showed the highest hemostatic efficiency and was found to be a promising biomaterial for the elaboration of a hemostatic product.