The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid

Abstract

Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a lefthanded helix. We further show that the contacts in the ring can be distorted to maintain key N-Nprotein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription. © 2013 SGM.