Metabolite profiling in complex biological matrices such as serum requires high-throughput technologies capable of accurate and reproducible quantitative analysis and detection of slight differences in metabolite concentrations. Gas chromatography-mass spectrometry (GC-MS) is widely used for characterizing the metabolome. This chapter summarizes the necessary preparatory steps required to profile the metabolome using GC-MS. While this chapter focuses on evaluating polar metabolites in serum samples, the methods can be adapted to quantify nonpolar metabolites in other biological matrices.
CITATION STYLE
Rattner, J., Farshidfar, F., & Bathe, O. F. (2019). Gas chromatography-mass spectrometry and analysis of the serum metabolomic profile through extraction and derivatization of polar metabolites. In Methods in Molecular Biology (Vol. 1928, pp. 235–249). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9027-6_13
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