DNA Paternity Testing with Two Mismatches: Our Experience

Abstract

In paternity testing, the adopted rule is to exclude paternity when mismatch at more than two STR loci is observed. Here we present two routine DNA paternity testing cases with two autosomal STR mismatches between the alleged father and female child, where we employed X-STR analysis to confirm or exclude paternity. Mismatches at D16S539 and D18S51 loci in the Case 1 and D8S1179 and FGA loci in the Case 2 were detected using PowerPlex® Fusion System. X-STR analysis using Investigator® Argus X-12 kit yielded one inconsistency at DXS10135 locus between the child and the alleged father in the Case 1, whereas we found a complete match at all 12 analysed X-STR loci in the Case 2. As a result, paternity was confirmed in both cases. Conclusively, this case study indicates that including an additional analysis has a great importance in solving paternity cases with inconclusive results of autosomal STR analysis.