Peripheral blood is the easy-to-access, minimally invasive, and the most abundant cell source to use for cell reprogramming. The episomal vector is among the best approaches for generating integration-free induced pluripotent stem (iPS) cells due to its simplicity and affordability. Here we describe the detailed protocol for the efficient generation of integration-free iPS cells from peripheral blood mononuclear cells. With this optimized protocol, one can readily generate hundreds of iPS cell colonies from 1 ml of peripheral blood.
CITATION STYLE
Su, R. J., Neises, A., & Zhang, X. B. (2016). Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors. In Methods in Molecular Biology (Vol. 1357, pp. 57–69). Humana Press Inc. https://doi.org/10.1007/7651_2014_139
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