Many biological processes are regulated by protein-protein interactions, and the analysis of these interactions has been a productive endeavor contributing to our understanding of cellular organization and function. The yeast two-hybrid technique is a widely used, powerful method of analyzing protein-protein interactions. The currently used formats, however, have inherent limitations, providing an opportunity to develop new alternatives that extend our ability to detect protein-protein interactions of biological relevance. Here we present a two-hybrid system named SRYTH (S te11p/Ste50p r elated y east t wo-h ybrid) based on the Ste11p/Ste50p interaction that uses the activation of the HOG pathway of Saccharomyces cerevisiae as a reporter for interactions. The system is suitable for detecting cytoplasmic protein interactions in their natural subcellular environment, and has been successfully used to investigate protein-protein interactions, including transcription factor associations, in Candida albicans.
CITATION STYLE
Mallick, J., Jansen, G., Wu, C., & Whiteway, M. (2016). SRYTH: A new yeast two-hybrid method. In Methods in Molecular Biology (Vol. 1356, pp. 31–41). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3052-4_3
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