Prolongation of the effective copulation period by fractionated-dose irradiation in the sweet potato weevil, Cylas formicarius

Abstract

The sterile insect technique (SIT), based on the principles of population and behavioral ecology, is widely used to suppress or eradicate target pest insect populations. The effectiveness of SIT depends on the ability of released sterile males to mate with and inseminate wild females; however, the use of gamma radiation to induce sterility negatively affects both somatic cells as well as reproductive cells. Consequently, sterilization by irradiation drastically diminishes mating performance over time. It is well known that fractionated-dose irradiation, in which a sterilizing dose is delivered via a series of smaller irradiations, reduces radiation damage. In the present study, we evaluated the effect of fractionated-dose irradiation on fertility, longevity, and mating propensity in Cylas formicarius (Summers) (Coleoptera: Brentidae) for 16days after irradiation. Fractionated-dose irradiation with 200Gy induced full sterility regardless of the number of radiation doses. Although the mating propensity of males sterilized by a single 200Gy dose (the current standard of the Okinawa Prefecture SIT program) was equal to that of non-irradiated weevils for the first 6days, the mating propensity of males sterilized by a series of three doses was maintained for at least the first 12days. These results demonstrated that fractionated-dose irradiation can be highly advantageous in C. formicarius eradication programs. © 2011 The Authors. Entomologia Experimentalis et Applicata © 2011 The Netherlands Entomological Society.