Glycoprofiling recombinant proteins expressed and secreted from mammalian cells is key to understanding their interactions with glycoprotein receptors in vivo. Recently, recombinant T cell receptors (TCRs) are being considered as therapeutic moieties. Here we present a mass spectrometry based protocol with a “bottom up” approach to characterize glycosylation in recombinant fusion proteins with α/β TCR constant domains expressed in mammalian cells. The protocol focuses on using peptide mass mapping and mass spectrometry for N-linked glycan profiling, including analyses of site occupancy, glycan heterogeneity, and possible glycan compositions and structures.
CITATION STYLE
Zhang, K., Demarest, S. J., Wu, X., & Fitchett, J. R. (2017). Glycosylation profiling of α/β T cell receptor constant domains expressed in mammalian cells. In Methods in Molecular Biology (Vol. 1575, pp. 197–213). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6857-2_12
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