Effects of long-term cold exposure on contractile properties in slow- and fast-twitch muscles of rats

Abstract

The effects of 20-week cold exposure on contractile properties of soleus and extensor digitorum longus (EDL) muscles and plasma hormone levels were studied in rats. Twenty male Wistar rats (5 week old) were randomly divided into 2 groups (n=10 each): cage-control and cold-exposed. The rats in the cold-exposed group were immersed in shoulder-deep water (∼18°C) for 1 h/d, 5 d/week, for 20 weeks. The temperature and humidity of the animal room with 12:12h light-dark cycle were maintained at ∼23°C and 55%, respectively. The rats were pair-fed powdered diets. The electromyogram activities in soleus and EDL were elevated by cold exposure. The body weight and absolute soleus wet weight of the cold-exposed group were significantly less than controls at the end of experiment. The one-half relaxation time and contraction time of EDL were significantly longer in the cold-exposed group than in the control group. The rate of twitch tension development, normalized by the maximum twitch tension, in EDL of the cold-exposed group was less than in the control group. Further, the fatigue resistance of EDL, but not of soleus, in response to train stimulation at 10 Hz was improved by cold exposure. The plasma levels of thyroid hormones, 3,5,3′-triiodothyronine and thyroxine, were significantly greater in cold-exposed group. Similar changes were also seen in the plasma catecholamine levels in the cold-exposed group (p>0.05). It is suggested that long-term cold exposure causes a shift of the contractile properties of fast-twitch EDL muscle toward the slow-twitch type. The results also indicated that the characteristics of muscles responded more strongly to an increased activity level than to the elevation of plasma hormones.