The polyphenol composition of Cistus incanus L., Trachystemon orientalis L. and Melissa offi cinalis L. infusions by HPLC-DAD method

  • Dimcheva V
  • Kaloyanov N
  • Karsheva M
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The user has requested enhancement of the downloaded file. v 031 Citation: Dimcheva V, Kaloyanov N, Karsheva M (2019) The polyphenol composition of Cistus incanus L., Trachystemon orientalis L. and Melissa officinalis L. infusions by HPLC-DAD method. Open J Anal Bioanal Chem 3(1): 031-038. DOI: Abstract Because of plants, health benefi ts consumption of herbal infusions as a dietary additive has increased in the last years. The aim of the present study was to establish the individual phenolic profi les using high-performance liquid chromatography with diode-array detection (HPLC-DAD) method of Bulgarian Cistus incanus leaves harvested in summer and winter seasons, Cistus incanus hard-coated seeds, Greek Cistus incanus leaves harvested in summer, Bulgarian Trachystemon orientalis, and Melissa offi cinalis leave and stems extracts prepared as infusions. Cistus incanus L. has played a very interesting role in the historical medicine of the Mediterranean region where is well known. Trachytsemon orientalis L. is wild borage typical for Caucasus nature. Both wild plants are widely distributed in Strandja mountain, Bulgaria and the ethnomedicinal description of them as anti-infl ammatory and antioxidant herbs are missing. The Melissa offi cinalis is wildly used as an aromatic, culinary and medicine remedy and its medicinal properties are well known of Bulgarian folk medicine. In the present study, the newly established HPLC-DAD method developed and validated previously for simultaneous separation of 9 natural substances common in plants-tree major catechins, four major fl avonoids, gallic and vanillic acid was used. The method was successfully applied to the determination of the specifi c bioactive substances in the investigated herbs and especially Cistus incanus species. Analyses under the method applied revealed that in the Bulgarian Cistus incanus summer leaves infusion, the values of (-)-epicatechin (3460.54 ± 1.89 μg/mL) and (-)-epigallocatechin (7369.23 ± 2.56 μg/mL) were found to be signifi cantly higher (p > 0.05) than values of the two catechins found in the other infusions. However, the winter Cistus incanus leaves contains 640.03 ± 1.58 μg/mL (-)-epigallocatechin and 489.98 ± 2.89 μg/mL rutin which are signifi cantly higher concentrations (p > 0.05) almost 2 times more than these present in the Greek Cistus incanus leaves infusion 386.69 ± 3.85 μg/mL and 273.07 ± 0.96 μg/mL, respectively. Nevertheless, the Greek Cistus incanus leaves contains the highest concentration of gallic acid (219.04 ± 1.06 μg/mL, p > 0.05) among all analyzed infusions. The highest polyphenol concentration found in Melissa offi cinalis leaves and stems infusion was (-)-epigallocatechin (188.99 ± 2.08 μg/mL) follow by quercetin, gallic acid, and kaempferol. In Trachystemon orientalis were found only the fl avonoids rutin (56.46 ± 0.31 μg/mL) and myricetin (630.23± 1.63 μg/mL). The investigation has led to a better understanding of the polyphenolic profi les of the analyzed plant infusions, especially the non-traditional for the Bulgaria herbs-Cistus incanus and Trachystemon orientalis and has clearly supported the further utilization of the two species in the Bulgarian traditional medicine. Not least the Cistus incanus leaves harvested in the winter season and Cistus incanus hard-coated seeds could be used as an antioxidant infusion with high added value.

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Dimcheva, V., Kaloyanov, N., & Karsheva, M. (2019). The polyphenol composition of Cistus incanus L., Trachystemon orientalis L. and Melissa offi cinalis L. infusions by HPLC-DAD method. Open Journal of Analytical and Bioanalytical Chemistry, 3(1), 031–038. https://doi.org/10.17352/ojabc.000008

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