Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
CITATION STYLE
Wefers, B., Brandl, C., Ortiz, O., Wurst, W., & Kühn, R. (2016). Genome Editing in Mice Using TALE Nucleases (pp. 229–243). https://doi.org/10.1007/978-1-4939-2932-0_17
Mendeley helps you to discover research relevant for your work.