Multiple antibiotics resistant among environmental isolates of Stenotrophomonas maltophilia

Abstract

In this study we assessed the functionality of integrons, melanin-like pigment and biofilm formation on multidrug resistance among environmental isolates of Stenotrophomonas maltophilia. Marked resistances were noted against aztreonam (60%), cefepime (68%), ceftazidime (77%), ciprofloxacin (72%), gentamicin (65%), meropenem (75%), piperacillin/tazobactam (65%) in S. maltophilia. Ticarcillin/clavulanic acid (66%) and trimethoprim/sulfamethoxazole (75%) were the active antibiotics against S. maltophilia. Class 1 intergron was significantly detected in 56.3% (54/96) of S. maltophilia strains. Integron-positive strains were significantly resistant to cefepime (69%), ceftazidime (78%), ciprofloxacin (74%), gentamicin (65%), and meropenem (72%). Gene cassettes arrays within integrons were identified as aminoglycoside resistance genes aacA4, aadA2, aadB, aacC4, and aacA6'-Ib; β-lactams resistance genes bla IMP , bla OXA , and bla CARB ; chloramphenicol resistance genes cmlA and catB2; quaternary ammonium compound (QAC) resistance genes smr and qac; and multi-gene cassettes: smr/aacA4 and bla IMP /aac6-II/aadA5. High-pigment-producing S. maltophilia strains revealed significant correlation with resistance to cefepime, ceftazidime, ciprofloxacin, levofloxacin and piperacillin/ tazobactam. Biofilm formation was not significant with resistance to ciprofloxacin, levofloxacin, meropenem, ticarcillin/clavulanic and trimethoprim /sulfamethoxazole. Our findings characterize the significant roles of integrons, melanin-like pigment and biofilm formation in the multidrug resistance of S. maltophilia. The range of antibiotics resistance genes and mobile genetic elements found suggests that the organism could potentially act as a reservoir of drug resistance determinants in environmental and clinical settings, which is an issue of public health concern.© JASEM http://dx.doi.org/10.4314/jasem.v18 i2.16 Introduction: Stenotrophomonas maltophilia (formerly known as Pseudomonas maltophilia, Xanthomonas maltophilia) is a ubiquitous species from the gamma subdivision of Proteobacteria (Hayward et al., 2010). S. maltophilia is a Gram-negative opportunistic pathogen, and increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality rate (Jumaa et al., 2006; Brooke, 2012). S. maltophilia is an environmental bacterium found in aqueous habitats, plant rhizospheres, animals, foods, contaminated medical care fluids, and water sources (Ryan et al., 2009). Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections (Brooke, 2012).