Whole cell protein profiling of human pathogenic bacteria isolated from clinical samples

Abstract

The present investigation deals with the protein profiling of clinical pathogens isolated from clinical samples. For the purpose, four different bacteria were isolated from the clinical samples and biochemically characterized. The 16S rRNA sequence analysis revealed the isolated bacteria were Escherichia coli, Staphylococcus epidermidis, Bacillus subtilis and Klebsiella pneumonia. The bacteria were cultured in liquid medium and the protein profile of the bacteria was investigated through ultra-sonication followed by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis. The results revealed that the unique protein bands observed in the bacteria at defined size can act as biomarker for the identification and differentiation of the bacteria from the other species.