Effect of culture media and temperature on growth and sporulation of Colletotrichum lindemuthianum of urdbean in vitro

Abstract

Anthracnose (Colletotrichum lindemuthianum) fungus is an important in the urdbean\rlegumes crop. In the present investigation, the rate of growth of Anthracnose (C.\rlindemuthianum) has been compared in various temperature, culture media solid and\rliquid while in case solid media types viz., simple PDA (Potato Dextrose Agar), Potato\rcarrot sucrose agar, Carrot juice agar, Blackgram leaf extract agar, Richard’s agar,\rCzapeck’s (Dox) agar, Asthana and Hawker’s agar had tested. Among all the solid\rmedia tested, maximum mycelial growth was obtained in Potato dextrose agar medium\r(80.04 mm), which was statistically at par with Richard’s agar medium (76.52 mm).Similarly\rin the liquid media, maximum dry mycelial weight was recorded in Richard’s broth\rmedium (444.34 mg) after 10 days of inoculation which was significantly superior over\rrest of the broth medias and followed potato dextrose agar broth (429.03 mg). Temperature\rplays an important role in infection and disease development. Maximum mean colony\rdiameter of fungus was recorded at temperatures of 30 0C (80.92 mm) and 25 0C (78.62\rmm) which was significantly superior over all other temperatures. Lowest mean colony\rdiameter was obtained at temperatures of 10 0C (24.01 mm) and 40 0C (21.37 mm). This is\rimportant for further study of disease management.