A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction

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Abstract

An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus bulgaricus, Lactobacillus helveticus, Leuconostoc mesanteroides, Listeria monocytogenes, Staphylococcus aureus, and Streptococcus salivarus subsp. thermophilus were amplified, cloned, and sequenced. The G+C contents of the DNA from these species range from 28 to 52%. The sequences of the bacterial recA genes show strong relatedness. This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning.

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Duwat, P., Ehrlich, S. D., & Gruss, A. (1992). A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction. Journal of Bacteriology. https://doi.org/10.1128/jb.174.15.5171-5175.1992

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