Cloning and characterization of argonaute genes in tomato

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Abstract

Argonaute (AGO) proteins are core elements in plant posttranscriptional RNA silencing pathways. The identification and functional characterization of tomato (Solanum lycopersicum) AGOs will help to better understand RNA silencing-based posttranscriptional regulation in fleshy fruits. Here we describe how to identify and clone SlAGO genes, as well as the methodology for their functional characterization.

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Xian, Z., Yan, F., & Li, Z. (2017). Cloning and characterization of argonaute genes in tomato. In Methods in Molecular Biology (Vol. 1640, pp. 191–197). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7165-7_13

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