Proteomic analysis of Arabidopsis root gravitropism

Abstract

In gravitropism of Arabidopsis root, the Arabidopsis root proteins induced and/or changed by gravitational stimulation were analyzed using a proteomic method. In this study, it was found that the levels of expression of some proteins related to the cytoskeleton and the calcium signal transmission system had changed during gravitational stimulation. In addition, the β subunit of the E1 component of pyruvate dehydrogenase, fructose bisphosphate aldolase, and 20S proteasome β subunit E1 protein appeared in two different molecular weight types during gravitational stimulation. The transitory changes in molecular weight in these three identified proteins were important findings although their functions in the gravity response in the roots of Arabidopsis seedlings remain uncertain. As far as we know, this is the first study involving the molecular weight shift-change in the same protein during the gravitropism response in plants using proteomic analysis.