PURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups. CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin GeneRelated Peptide (15 kDa) neuropeptides in rat skin.
CITATION STYLE
Raymundo, E. C., Hochman, B., Nishioka, M. A., de Freitas, J. O. G., Maximino, J. R., Chadi, G., & Ferreira, L. M. (2014). Effects of subcutaneous carbon dioxide on Calcitonin gene related peptide and substance P secretion in rat skin. Acta Cirurgica Brasileira, 29(4), 224–230. https://doi.org/10.1590/s0102-86502014000400002
Mendeley helps you to discover research relevant for your work.