MicroRNAs are key regulators in the development processes or stress responses in plants. In the last decade, several conserved or non-conserved microRNAs have been identified in Medicago truncatula. Different strategies leading to the inactivation of microRNAs in plants have been described. Here, we propose a protocol for an effective inactivation of microRNAs using a STTM strategy in M. truncatula transgenic roots.
CITATION STYLE
Proust, H., Bazin, J., Sorin, C., Hartmann, C., Crespi, M., & Lelandais-Brière, C. (2018). Stable inactivation of microRNAs in Medicago truncatula roots. In Methods in Molecular Biology (Vol. 1822, pp. 123–132). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8633-0_9
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